Comparative Highly Efficient Production of β-glucan by Lasiodiplodia theobromae CCT 3966 and Its Multiscale Characterization

Lasiodiplodan, a (1→6)-β-d-glucan, is an exopolysaccharide with high commercial value and many applications in food, pharmaceuticals, cosmetics. Current industrial production of β-glucans from crops mostly by chemical routes generating hazardous toxic waste. Therefore, alternative sustainable eco-friendly pathways are highly desirable. Here, we have studied the lasiodiplodan sugarcane bagasse (SCB), major lignocellulosic agricultural residue, Lasiodiplodia theobromae CCT 3966. Lasiodiplodan accumulated on SCB hydrolysate (carbon source) supplemented soybean bran or rice (nitrogen was 16.2 [6.8 × 103 Da] 22.0 [7.6 g/L, respectively. showed purity, low solubility, pseudoplastic behavior composed glucose units. Moreover, exopolysaccharides were substantially amorphous moderate thermal stability similar degradation temperatures. To our knowledge, this first report highest SCB-based to date. L. theobromae, as microbial cell factory, demonstrated potential for renewable biomass feedstock.